Construction of cDNA library
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Construction of cDNA library
3.6K viewsJul 10, 2024
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Brianna Bibel on Instagram: "When you order a cDNA plasmid (the DNA copy of the mature messenger RNA (mRNA) instructions for making a protein) from a repository (e.g. addgene, DNASU), it will likely come in some sort of generic cloning plasmid. And you will want to “subclone” it into an expression plasmid if you want to use it to make protein from it (i.e. move it out of the one plasmid and into another). https://youtu.be/ws6C8X7reYs blog form: https://bit.ly/vector_plasmids ; longer video: http
9:20
Brianna Bibel on Instagram: "When you order a cDNA plasmid (the DNA copy of the mature messenger RNA (mRNA) instructions for making a protein) from a repository (e.g. addgene, DNASU), it will likely come in some sort of generic cloning plasmid. And you will want to “subclone” it into an expression plasmid if you want to use it to make protein from it (i.e. move it out of the one plasmid and into another). https://youtu.be/ws6C8X7reYs blog form: https://bit.ly/vector_plasmids ; longer video: http
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